Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 titration25KG
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio1KG
TAPS BioXtra 99.5 titratio1KG
Sigma Aldrich Fine Chemicals Biosciences MOPSO >=99% (titration) | 68399-77-9 | MFCD00009629 | 100G
MOPSO >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 225.26 | 68399-77-9 | MFCD00009629 | 100G
Biotang Inc Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml
Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml.
Biotang Inc HEPES-Triton Protein Extraction Buffer, 250 ml
HEPES-Triton Protein Extraction Buffer, 250 ml.
Biotang Inc Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml
Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml.
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
Fluidigm Corp Maxpar PBS, 500 mL
Formulated to provide the best possible signal to noise ratio for detection by mass cytometry.
Biotang Inc TRIS-Buffered Saline (10X, Low Salt), 1000 ml
TRIS-Buffered Saline (10X, Low Salt), 1000 ml.