Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbecco’s Phosphate Buffered Saline, 1000 mL
Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process, where the presence of calcium and magnesium can inhibit trypsin activity. For this application, use DPBS Modified, which is formulated without calcium and magnesium salts.
Quality Biological Inc TAE Buffer 50X, 4 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 20 Liters
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Hach Company pPb-1 Acid Preservative Solution, 236 mL
For Lead determination by the LeadTrak™ Fast Column Extraction method. Hach method 8317.
Hampton Research HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml
HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Boston Bioproducts Inc Phosphate Buffered Saline-Tween (PBST), 0.05% Tween-20, pH 7.4, 7.0 - 7.4 buffer range, ready-to-use, immunoassays, ELISA, Western Blot, protein purification
Phosphate Buffered Saline-Tween (PBST), 0.05% Tween-20, pH 7.4, 7.0 - 7.4 buffer range, ready-to-use, immunoassays, ELISA, Western Blot, protein purification
TRIBIOSCIENCE INC 2x HBS, pH 7.05 (Sterile, Tissue Culture Grade) 500 ML
Tribioscience’s HBS (2x HEPES buffered saline) is ready to use for chemical transfection, including calcium phosphate transfection and purposes such as calcium transfection, calcium phosphate transfection, mammalian cell transfection, virus production, lentivirus and retrovirus transfection, and AAV transfection.
Hampton Research HR2-785/1.0 M HEPES pH 7.0 - 100 ml
HR2-785/1.0 M HEPES pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Biotang Inc Phosphate-buffered saline (PBS, 10X), 2000ml
Phosphate-buffered saline (PBS, 10X), 2000ml.